Journal: Antibiotics

Article Title: Hyperbranched Polylysine Exhibits a Collaborative Enhancement of the Antibiotic Capacity to Kill Gram-Negative Pathogens

doi: 10.3390/antibiotics13030217

Figure Lengend Snippet: Fluorescence values obtained for the NPN uptake assay of E. coli O157:H7 following the addition of HBPL-6 in the range of 0.78–100 µg/mL.

Article Snippet: The following pathogenic bacteria were used in the tests: Salmonella typhimurium (CICC 22956, China Industrial Microbial Strain Preservation and Management Centre), Escherichia coli O157:H7 (CICC 10907, China Industrial Microbial Strain Collection Management Centre, Beijing, China), and Pseudomonas aeruginosa PAO1 (ATCC 27853, American Type Culture Collection, Littleton, CO, USA).

Techniques: Fluorescence

Journal: Antibiotics

Article Title: Hyperbranched Polylysine Exhibits a Collaborative Enhancement of the Antibiotic Capacity to Kill Gram-Negative Pathogens

doi: 10.3390/antibiotics13030217

Figure Lengend Snippet: NPN fluorescence values of HBPL-6 compared with ε-PL. ( a – c , respectively) E. coli O157:H7, S. typhimurium , and P. aeruginosa PAO1 treated with the addition of different concentrations (0.78–3.125 µg/mL) of HBPL-6 and ε-PL. (n.s.: not significant; *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001).

Article Snippet: The following pathogenic bacteria were used in the tests: Salmonella typhimurium (CICC 22956, China Industrial Microbial Strain Preservation and Management Centre), Escherichia coli O157:H7 (CICC 10907, China Industrial Microbial Strain Collection Management Centre, Beijing, China), and Pseudomonas aeruginosa PAO1 (ATCC 27853, American Type Culture Collection, Littleton, CO, USA).

Techniques: Fluorescence

Journal: Antibiotics

Article Title: Hyperbranched Polylysine Exhibits a Collaborative Enhancement of the Antibiotic Capacity to Kill Gram-Negative Pathogens

doi: 10.3390/antibiotics13030217

Figure Lengend Snippet: The assessment of outer-membrane permeabilization by the HBPL-6 of G − bacteria. ( a – c ) PI fluorescence intensities of the additions of different concentrations of HBPL-6 (0.78–100 µg/mL) for the 30 min incubation periods of E. coli O157:H7, S. typhimurium , and P. aeruginosa PAO1, respectively. ( d – f , respectively) Presence of 260 nm absorbing materials in the supernatants of E. coli O157:H7, S. typhimurium , and P. aeruginosa PAO1 treated with HBPL-6 (0.78–100 µg/mL). ( g – i , respectively) Total ROS accumulation values for E. coli O157:H7, S. typhimurium , and P. aeruginosa PAO1 treated with HBPL-6 (0.78–100 µg/mL). The data are the average triplicates using a non-parametric one-way ANOVA test. (n.s.: not significant; *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001).

Article Snippet: The following pathogenic bacteria were used in the tests: Salmonella typhimurium (CICC 22956, China Industrial Microbial Strain Preservation and Management Centre), Escherichia coli O157:H7 (CICC 10907, China Industrial Microbial Strain Collection Management Centre, Beijing, China), and Pseudomonas aeruginosa PAO1 (ATCC 27853, American Type Culture Collection, Littleton, CO, USA).

Techniques: Membrane, Bacteria, Fluorescence, Incubation

Journal: Antibiotics

Article Title: Hyperbranched Polylysine Exhibits a Collaborative Enhancement of the Antibiotic Capacity to Kill Gram-Negative Pathogens

doi: 10.3390/antibiotics13030217

Figure Lengend Snippet: Minimum inhibitory concentrations of HBPL-6 for representative Gram-negative bacteria.

Article Snippet: The following pathogenic bacteria were used in the tests: Salmonella typhimurium (CICC 22956, China Industrial Microbial Strain Preservation and Management Centre), Escherichia coli O157:H7 (CICC 10907, China Industrial Microbial Strain Collection Management Centre, Beijing, China), and Pseudomonas aeruginosa PAO1 (ATCC 27853, American Type Culture Collection, Littleton, CO, USA).

Techniques: Bacteria

Journal: Antibiotics

Article Title: Hyperbranched Polylysine Exhibits a Collaborative Enhancement of the Antibiotic Capacity to Kill Gram-Negative Pathogens

doi: 10.3390/antibiotics13030217

Figure Lengend Snippet: Plots were generated using the Combenefit program for the synergistic potentiation result for the use of HBPL-6 on erythromycin. ( a ) E. coli O157:H7 with HBPL-6 (0–100 μg/mL) and erythromycin (0–128 μg/mL). ( b ) S. typhimurium with HBPL-6 (0–100 μg/mL) and erythromycin (0–256 μg/mL). ( c ) P. aeruginosa PAO1 with HBPL-6 (0–100 μg/mL) and erythromycin (0–1024 μg/mL). Growth curves for single- and dual-drug combinations ( d , g , respectively): E. coli O157:H7 with 6.25 and 12.5 μg/mL dose administrations of HBPL-6 and 8 μg/mL of erythromycin. ( e , h ) S. typhimurium with 6.25 and 25 μg/mL dose administrations of HBPL-6 and 16 μg/mL of erythromycin. ( f , i ) P. aeruginosa PAO1 with 12.5 and 25 μg/mL dose administrations of HBPL-6 and 16 μg/mL of erythromycin.

Article Snippet: The following pathogenic bacteria were used in the tests: Salmonella typhimurium (CICC 22956, China Industrial Microbial Strain Preservation and Management Centre), Escherichia coli O157:H7 (CICC 10907, China Industrial Microbial Strain Collection Management Centre, Beijing, China), and Pseudomonas aeruginosa PAO1 (ATCC 27853, American Type Culture Collection, Littleton, CO, USA).

Techniques: Generated

Journal: Antibiotics

Article Title: Hyperbranched Polylysine Exhibits a Collaborative Enhancement of the Antibiotic Capacity to Kill Gram-Negative Pathogens

doi: 10.3390/antibiotics13030217

Figure Lengend Snippet: Plots were generated using the Combenefit program for the synergistic potentiation result of the use of HBPL-6 on rifampin. ( a ) E. coli O157:H7 with HBPL-6 (0–100 μg/mL) and rifampin (0–128 μg/mL). ( b ) S. typhimurium with HBPL-6 (0–100 μg/mL) and rifampin (0–64 μg/mL). ( c ) P. aeruginosa PAO1 with HBPL-6 (0–100 μg/mL) and rifampin (0–256 μg/mL). Growth curves for single- and dual-drug combinations ( d , g , respectively): E. coli O157:H7 with 6.25 and 12.5 μg/mL doses of HBPL-6 and 8 μg/mL of rifampin. ( e , h ) S. typhimurium with 6.25 and 12.5 μg/mL doses of HBPL-6 and 2 μg/mL of rifampin. ( f , i ) P. aeruginosa PAO1 with 6.25 and 25 μg/mL doses of HBPL-6 and 16 μg/mL of rifampin.

Article Snippet: The following pathogenic bacteria were used in the tests: Salmonella typhimurium (CICC 22956, China Industrial Microbial Strain Preservation and Management Centre), Escherichia coli O157:H7 (CICC 10907, China Industrial Microbial Strain Collection Management Centre, Beijing, China), and Pseudomonas aeruginosa PAO1 (ATCC 27853, American Type Culture Collection, Littleton, CO, USA).

Techniques: Generated

Journal: Antibiotics

Article Title: Hyperbranched Polylysine Exhibits a Collaborative Enhancement of the Antibiotic Capacity to Kill Gram-Negative Pathogens

doi: 10.3390/antibiotics13030217

Figure Lengend Snippet: Plots were generated using the Combenefit program for the synergistic potentiation result for the use of HBPL-6 on colistin. ( a ) E. coli O157:H7 with HBPL-6 (0–100 μg/mL) and colistin (0–16 μg/mL). ( b ) S. typhimurium with HBPL-6 (0–100 μg/mL) and colistin (0–16 μg/mL). ( c ) P. aeruginosa PAO1 with HBPL-6 (0–100 μg/mL) and colistin (0–16 μg/mL). Growth curves for single- and dual-drug combinations ( d , g , respectively): E. coli O157:H7 with 3.125 and 6.25 μg/mL doses of HBPL-6 and 0.25 μg/mL of colistin. ( e , h ) S. typhimurium with 0.78 and 1.56 μg/mL doses of HBPL-6 and 2 μg/mL of colistin. ( f , i ) P. aeruginosa PAO1 with 6.25 and 12.5 μg/mL doses of HBPL-6 and 1 μg/mL of colistin.

Article Snippet: The following pathogenic bacteria were used in the tests: Salmonella typhimurium (CICC 22956, China Industrial Microbial Strain Preservation and Management Centre), Escherichia coli O157:H7 (CICC 10907, China Industrial Microbial Strain Collection Management Centre, Beijing, China), and Pseudomonas aeruginosa PAO1 (ATCC 27853, American Type Culture Collection, Littleton, CO, USA).

Techniques: Generated

Journal: BMC Complementary and Alternative Medicine

Article Title: Spermicidal and anti- Trichomonas vaginalis activity of Brazilian Sapindus saponaria

doi: 10.1186/1472-6882-13-196

Figure Lengend Snippet: Susceptibility of Trichomonas vaginalis to water-ethanol (WE) and butanolic (BE) extracts, and saponins (SP) of Sapindus saponaria

Article Snippet: The compounds were effective against Trichomonas vaginalis (Minimum Inhibitory Concentration = 0.156 mg/mL for WE and BE, and 0.078 mg/mL for SP against a clinical strain (CS); and 0.312, 0.156 and 0.078 mg/mL for WE, BE and SP, respectively, against an ATCC strain).

Techniques:

Journal: Cell

Article Title: Large-Scale Analyses of Human Microbiomes Reveal Thousands of Small, Novel Genes

doi: 10.1016/j.cell.2019.07.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Microbe strains The bacterial strain used in this study is Bacteroides thetaiotaomicron VPI-5482 (ATCC 29148).

Techniques: RNA HS Assay, DNA HS Assay, Sequencing, Software